One of the biggest differences between an online presentation and a face-to-face meeting is the ability to recognize body language and facial expressions. If you keep an eye on the facial expressions and body language of the participants, you can recognize much earlier whether the participant expresses agreement or disagreement.
For this, however, it is necessary to have the participant videos always in view. The position of the participant videos and the own camera should be placed in a line, e.g. at the top in the middle, so that you can look the participants in the eyes.
A typical negative example is that the video and camera are on monitor 1 and the presentation you are looking at is on monitor 2. This causes you to talk to the participants with your head turned away – neither elegant nor polite.
The possibilities to score points with your own facial expressions and body language are very close to an on-site conversation.